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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
06/09/2021 |
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Actualizado : |
25/04/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
CLAVIJO, F.; CURLAND, R.D.; CROCE, V.; LAPAZ, M.I.; DILL-MACKY, R.; PEREYRA, S.; SIRI, M.I. |
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Afiliación : |
FELIPE CLAVIJO,, Universidad de la República Facultad de Química, 201894, Área Microbiología, Departamento de Biociencias, Montevideo, Montevideo, Uruguay.; REBECCA D. CURLAND,, University of Minnesota System, 311816, Plant Pathology, Minneapolis, Minnesota, United States.; VALENTINA CROCE, Universidad de la República Facultad de Química, 201894, Área Microbiología, Departamento de Biociencias, Montevideo, Montevideo, Uruguay.; MARÍA INÉS LAPAZ, Universidad de la República Facultad de Química, 201894, Área Microbiología, Departamento de Biociencias, Montevideo, Montevideo, Uruguay.; RUTH DILL-MACKY, University of Minnesota System, 311816, Plant Pathology, Minneapolis, Minnesota, United States.; SILVIA ANTONIA PEREYRA CORREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA INÉS INÉS SIRI, Universidad de la República Facultad de Química, 201894, Área Microbiología, Departamento de Biociencias, Montevideo, Montevideo, Uruguay. |
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Título : |
Genetic and phenotypic characterization of Xanthomonas species pathogenic of wheat in Uruguay. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Phytopathology, March 2022, vol. 112 (3): 511-520. Doi: https://doi.org/10.1094/PHYTO-06-21-0231-R |
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ISSN : |
e-ISSN:1943-7684 |
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DOI : |
10.1094/PHYTO-06-21-0231-R |
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Idioma : |
Inglés |
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Notas : |
Article history: Accepted for publication/ Posted online on 12 Aug 2021, First Look. |
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Contenido : |
Abstract: Bacterial diseases affecting wheat production in Uruguay are an issue of growing concern yet remain largely uninvestigated in the region. Surveys of 61 wheat fields carried out from 2017 to 2019 yielded a regional collection of 63 strains identified by 16S rRNA gene analysis as Xanthomonas spp. A real-time PCR protocol using species-specific primers previously reported allowed the identification of 44 strains as X. translucens, the causal agent of bacterial leaf streak (BLS) in wheat and other cereal crops. Multilocus sequence analysis (MLSA) of four housekeeping genes (dnaK, fyuA, gyrB, and rpoD) revealed that these strains were most closely related to X. translucens pv. undulosa, the pathovar that is most commonly associated with BLS of wheat. Multilocus sequence typing (MLST) was applied to examine the genetic diversity among X. translucens strains. Strains were assigned to four different sequence types, three of which have been previously reported globally. Additionally, 17 Xanthomonas strains not belonging to X. translucens were obtained from diseased wheat leaves. Phylogenetic analysis showed that these strains are closely related to Xanthomonas prunicola, and clustered together with previously uncharacterized Xanthomonas strains isolated from wheat in Minnesota, US. In planta pathogenicity assays carried out on a BLS susceptible wheat cultivar showed that X. translucens pv. undulosa strains caused brown necrosis symptoms typical of BLS, while non-translucens Xanthomonas sp. strains elicited an atypical symptom of dry necrosis. These findings suggest that local wheat fields are affected by X. translucens pv. undulosa, and by a new wheat pathogen within the Xanthomonas genus. MenosAbstract: Bacterial diseases affecting wheat production in Uruguay are an issue of growing concern yet remain largely uninvestigated in the region. Surveys of 61 wheat fields carried out from 2017 to 2019 yielded a regional collection of 63 strains identified by 16S rRNA gene analysis as Xanthomonas spp. A real-time PCR protocol using species-specific primers previously reported allowed the identification of 44 strains as X. translucens, the causal agent of bacterial leaf streak (BLS) in wheat and other cereal crops. Multilocus sequence analysis (MLSA) of four housekeeping genes (dnaK, fyuA, gyrB, and rpoD) revealed that these strains were most closely related to X. translucens pv. undulosa, the pathovar that is most commonly associated with BLS of wheat. Multilocus sequence typing (MLST) was applied to examine the genetic diversity among X. translucens strains. Strains were assigned to four different sequence types, three of which have been previously reported globally. Additionally, 17 Xanthomonas strains not belonging to X. translucens were obtained from diseased wheat leaves. Phylogenetic analysis showed that these strains are closely related to Xanthomonas prunicola, and clustered together with previously uncharacterized Xanthomonas strains isolated from wheat in Minnesota, US. In planta pathogenicity assays carried out on a BLS susceptible wheat cultivar showed that X. translucens pv. undulosa strains caused brown necrosis symptoms typical of BLS, while non-translucens... Presentar Todo |
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Palabras claves : |
Bacterial leaf streak; Bacterial Pathogens; Multilocus sequence analysis and typing; POPULATION BIOLOGY; WHEAT; Xanthomonas. |
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Thesagro : |
FITOPATOLOGIA; TRIGO; TRITICUM AESTIVUM; URUGUAY. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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Marc : |
LEADER 02834naa a2200349 a 4500
001 1062388
005 2022-04-25
008 2022 bl uuuu u00u1 u #d
022 $ae-ISSN:1943-7684
024 7 $a10.1094/PHYTO-06-21-0231-R$2DOI
100 1 $aCLAVIJO, F.
245 $aGenetic and phenotypic characterization of Xanthomonas species pathogenic of wheat in Uruguay.$h[electronic resource]
260 $c2022
500 $aArticle history: Accepted for publication/ Posted online on 12 Aug 2021, First Look.
520 $aAbstract: Bacterial diseases affecting wheat production in Uruguay are an issue of growing concern yet remain largely uninvestigated in the region. Surveys of 61 wheat fields carried out from 2017 to 2019 yielded a regional collection of 63 strains identified by 16S rRNA gene analysis as Xanthomonas spp. A real-time PCR protocol using species-specific primers previously reported allowed the identification of 44 strains as X. translucens, the causal agent of bacterial leaf streak (BLS) in wheat and other cereal crops. Multilocus sequence analysis (MLSA) of four housekeeping genes (dnaK, fyuA, gyrB, and rpoD) revealed that these strains were most closely related to X. translucens pv. undulosa, the pathovar that is most commonly associated with BLS of wheat. Multilocus sequence typing (MLST) was applied to examine the genetic diversity among X. translucens strains. Strains were assigned to four different sequence types, three of which have been previously reported globally. Additionally, 17 Xanthomonas strains not belonging to X. translucens were obtained from diseased wheat leaves. Phylogenetic analysis showed that these strains are closely related to Xanthomonas prunicola, and clustered together with previously uncharacterized Xanthomonas strains isolated from wheat in Minnesota, US. In planta pathogenicity assays carried out on a BLS susceptible wheat cultivar showed that X. translucens pv. undulosa strains caused brown necrosis symptoms typical of BLS, while non-translucens Xanthomonas sp. strains elicited an atypical symptom of dry necrosis. These findings suggest that local wheat fields are affected by X. translucens pv. undulosa, and by a new wheat pathogen within the Xanthomonas genus.
650 $aFITOPATOLOGIA
650 $aTRIGO
650 $aTRITICUM AESTIVUM
650 $aURUGUAY
653 $aBacterial leaf streak
653 $aBacterial Pathogens
653 $aMultilocus sequence analysis and typing
653 $aPOPULATION BIOLOGY
653 $aWHEAT
653 $aXanthomonas
700 1 $aCURLAND, R.D.
700 1 $aCROCE, V.
700 1 $aLAPAZ, M.I.
700 1 $aDILL-MACKY, R.
700 1 $aPEREYRA, S.
700 1 $aSIRI, M.I.
773 $tPhytopathology, March 2022, vol. 112 (3): 511-520. Doi: https://doi.org/10.1094/PHYTO-06-21-0231-R
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INIA La Estanzuela (LE) |
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
23/11/2021 |
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Actualizado : |
06/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Nacionales |
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Circulación / Nivel : |
Nacional - -- |
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Autor : |
LADO, J.; MOLTINI, A.; PINTOS, P.; LUQUE, E.; GONCALVEZ, L.; RIVAS, F.; ALCAIRE, F.; ARES, G. |
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Afiliación : |
JOANNA LADO LINDNER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA INÉS MOLTINI PALADINO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PEDRO MAXIMILIANO PINTOS RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MAYZA ELEANA LUQUE NUÑEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA LUCIA GONCALVEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS FERNANDO RIVAS GRELA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FLORENCIA ALCAIRE, Universidad de la República, Facultad de Química, Polo Tecnológico de Pando, Sensometría y Ciencia del Consumidor, Canelones, Uruguay.; GASTÓN ARES, Universidad de la República, Facultad de Química, Polo Tecnológico de Pando, Sensometría y Ciencia del Consumidor, Canelones, Uruguay. |
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Título : |
Unraveling factors affecting consumers' liking of novel Uruguayan mandarins. [Descifrando los factores que afectan la aceptabilidad de nuevas mandarinas Uruguayas]. [Descifrando os fatores que afetam a aceitabilidade de novas tangerinas uruguaias]. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Agrociencia Uruguay, 2021, vol. 25, n.2, article E540. DOI: https://doi.org/10.31285/AGRO.25.540 |
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ISSN : |
2730-5066 |
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DOI : |
10.31285/AGRO.25.540 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 10 Feb 2021; Accepted 3 Aug 2021; Published 18 Aug 2021.
Editor: Gustavo González-Neves, Universidad de la República, Montevideo, Uruguay.
Correspondence: Joanna Lado, mail: jlado@inia.org.uy |
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Contenido : |
ABSTRACT - Mandarin cultivars show a great diversity of fruit-quality and sensory characteristics, together with an extended harvest season. Citrus breeding is focused on exploiting season niches for higher prices as well as nutritional and sensory aspects, among others. In this context, the sensory characterization of new hybrids and the identi-fication of key characteristics of consumers ? liking provide valuable information towards breeding efforts and marketing strategies. Previous works showed that sensory characteristics, and specially flavor, play a key role in consumer acceptance of mandarins. Two studies were carried out during mandarin harvest season (mid and late) applying ?check all that apply? (CATA) questions with at least 100 consumers. Overall liking scores of the most liked hybrids and cultivars ranged between 6.5 to 7.7 in the 9-point hedonic scale, evidencing a positive hedonic reaction. Local hybrids showed similar or higher values than the reference cultivars Tango, Gold Nugget, Murcott and Ortanique. Results showed that orange color, regular shape, smoothness, sweetness, juiciness, int ense and typical flavor are the key drivers of consumer's liking of mandarins, and that external appearance is not a good predictor of mandarin tasting experience. Hybrids F3P8, F5P8 and F2P3 were described using terms related to positive sensory characteristics and received liking scores similar or higher to reference cultivars such as Tango, stressing the importance of sensory and consumer science as an integral part of breeding strategies.
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RESUMEN - Las mandarinas presentan una gran diversidad en características sensoriales y de calidad de fruta, así como una cosecha extendida. El mejoramiento genético de cítricos se focaliza en la búsqueda de nichos de cosecha para la obtención de mayores precios, así como en aspectos nutricionales y sensoriales, entre otras caracterís-ticas. En este contexto, la caracterización sensorial de nuevos híbridos y la identificación de las características sensoriales claves que influyen en la aceptabilidad de los consumidores proveen de una valiosa información para el mejoramiento y las estrategias de marketing. Estudios previos mostraron que las características senso-riales, especialmente el sabor, son claves en la aceptación de las mandarinas por los consumidores. Se llevaron a cabo dos estudios durante la estación de cosecha de mandarinas (media y tardía) aplicando la metodología «marque todo lo que corresponda» (CATA) y con la participación de al menos 100 consumidores. La aceptabi-lidad de los cultivares que fueron más aceptados se situó entre 6,5 y 7,7 en una escala hedónica de 9 puntos, evidenciando una reacción hedónica positiva. Los nuevos híbridos presentaron puntajes de aceptabilidad simi-lares o superiores a los cultivares de referencia Tango, Gold Nugget, Murcott y Ortanique. A su vez, los resul-tados mostraron que el color naranja, la forma regular, la piel lisa, el dulzor, la jugosidad, el sabor intenso y el sabor típico a mandarina son características claves que determinan la aceptabilidad de las mandarinas, y que la apariencia externa no es un buen predictor del sabor. Los híbridos F3P8, F5P8 y F2P3 fueron descritos por los consumidores con términos sensoriales positivos, recibiendo valores de aceptabilidad similares o mayores que los cultivares de referencia como tango, reafirmando la importancia de la incorporación de estudios senso-riales en el contexto del mejoramiento genético.
.-.-.-.-.-.-.-.-.-.-.-.-.-.-.
RESUMO - As cultivares de tangerina apresentam grande diversidade na qualidade dos frutos e nas características senso-riais, juntamente com uma época de colheita prolongada. O melhoramento cítrico tem como foco a exploração de nichos sazonais por preços mais elevados, além de aspectos nutricionais e sensoriais, entre outros. Nesse contexto, a caracterização sensorial de diferentes híbridos e a identificação das características-chave do gosto do consumidor fornecem informações valiosas para os esforços de melhoramento e estratégias de marketing. Trabalhos anteriores mostraram que as características sensoriais e principalmente o sabor desempenham um papel fundamental na aceitação das tangerinas pelo consumidor. Dois estudos foram realizados durante a safra de tangerina (meio e final) aplicando perguntas do tipo cheque todos que se aplicam (CATA) com pelo menos 100 consumidores. As pontuações de aceitação geral dos híbridos e cultivares mais apreciados variaram entre 6,5 a 7,7 na escala hedônica de 9 pontos, evidenciando uma reação hedônica positiva. Os híbridos locais apresentaram valores semelhantes ou superiores aos cultivares de referência Tango, Gold Nugget, Murcott e Ortanique. Os resultados mostraram que a cor laranja, formato regular, maciez, doçura, sabor intenso y sabor tangerina e suculência típicos são os principais motivadores do gosto do consumidor por tangerinas e que a aparência externa não é um bom indicador da experiência de degustação da tangerina. Os híbridos F3P8, F5P8 e F2P3 foram descritos usando termos relacionados a características sensoriais positivas e receberam pontu-ações de gosto semelhantes ou superiores a cultivares de referência como o Tango, enfatizando a importância da ciência sensorial e do consumidor como parte integrante das estratégias de melhoramento. MenosABSTRACT - Mandarin cultivars show a great diversity of fruit-quality and sensory characteristics, together with an extended harvest season. Citrus breeding is focused on exploiting season niches for higher prices as well as nutritional and sensory aspects, among others. In this context, the sensory characterization of new hybrids and the identi-fication of key characteristics of consumers ? liking provide valuable information towards breeding efforts and marketing strategies. Previous works showed that sensory characteristics, and specially flavor, play a key role in consumer acceptance of mandarins. Two studies were carried out during mandarin harvest season (mid and late) applying ?check all that apply? (CATA) questions with at least 100 consumers. Overall liking scores of the most liked hybrids and cultivars ranged between 6.5 to 7.7 in the 9-point hedonic scale, evidencing a positive hedonic reaction. Local hybrids showed similar or higher values than the reference cultivars Tango, Gold Nugget, Murcott and Ortanique. Results showed that orange color, regular shape, smoothness, sweetness, juiciness, int ense and typical flavor are the key drivers of consumer's liking of mandarins, and that external appearance is not a good predictor of mandarin tasting experience. Hybrids F3P8, F5P8 and F2P3 were described using terms related to positive sensory characteristics and received liking scores similar or higher to reference cultivars such as Tango, stressing th... Presentar Todo |
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Palabras claves : |
Breeding; Calidad de fruta; Citros; Flavor; Fruit quality; Melhoramento; PLATAFORMA AGROALIMENTOS; Qualidade do fruto. |
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Thesagro : |
CITRUS; FITOMEJORAMIENTO; SABOR. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16731/1/Agrociencia-UY-2021-25-540.pdf
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Marc : |
LEADER 06892naa a2200373 a 4500
001 1062540
005 2022-09-06
008 2021 bl uuuu u00u1 u #d
022 $a2730-5066
024 7 $a10.31285/AGRO.25.540$2DOI
100 1 $aLADO, J.
245 $aUnraveling factors affecting consumers' liking of novel Uruguayan mandarins. [Descifrando los factores que afectan la aceptabilidad de nuevas mandarinas Uruguayas]. [Descifrando os fatores que afetam a aceitabilidade de novas tangerinas uruguaias].$h[electronic resource]
260 $c2021
500 $aArticle history: Received 10 Feb 2021; Accepted 3 Aug 2021; Published 18 Aug 2021. Editor: Gustavo González-Neves, Universidad de la República, Montevideo, Uruguay. Correspondence: Joanna Lado, mail: jlado@inia.org.uy
520 $aABSTRACT - Mandarin cultivars show a great diversity of fruit-quality and sensory characteristics, together with an extended harvest season. Citrus breeding is focused on exploiting season niches for higher prices as well as nutritional and sensory aspects, among others. In this context, the sensory characterization of new hybrids and the identi-fication of key characteristics of consumers ? liking provide valuable information towards breeding efforts and marketing strategies. Previous works showed that sensory characteristics, and specially flavor, play a key role in consumer acceptance of mandarins. Two studies were carried out during mandarin harvest season (mid and late) applying ?check all that apply? (CATA) questions with at least 100 consumers. Overall liking scores of the most liked hybrids and cultivars ranged between 6.5 to 7.7 in the 9-point hedonic scale, evidencing a positive hedonic reaction. Local hybrids showed similar or higher values than the reference cultivars Tango, Gold Nugget, Murcott and Ortanique. Results showed that orange color, regular shape, smoothness, sweetness, juiciness, int ense and typical flavor are the key drivers of consumer's liking of mandarins, and that external appearance is not a good predictor of mandarin tasting experience. Hybrids F3P8, F5P8 and F2P3 were described using terms related to positive sensory characteristics and received liking scores similar or higher to reference cultivars such as Tango, stressing the importance of sensory and consumer science as an integral part of breeding strategies. .-.-.-.-.-.-.-.-.-.-.-.-.-.-. RESUMEN - Las mandarinas presentan una gran diversidad en características sensoriales y de calidad de fruta, así como una cosecha extendida. El mejoramiento genético de cítricos se focaliza en la búsqueda de nichos de cosecha para la obtención de mayores precios, así como en aspectos nutricionales y sensoriales, entre otras caracterís-ticas. En este contexto, la caracterización sensorial de nuevos híbridos y la identificación de las características sensoriales claves que influyen en la aceptabilidad de los consumidores proveen de una valiosa información para el mejoramiento y las estrategias de marketing. Estudios previos mostraron que las características senso-riales, especialmente el sabor, son claves en la aceptación de las mandarinas por los consumidores. Se llevaron a cabo dos estudios durante la estación de cosecha de mandarinas (media y tardía) aplicando la metodología «marque todo lo que corresponda» (CATA) y con la participación de al menos 100 consumidores. La aceptabi-lidad de los cultivares que fueron más aceptados se situó entre 6,5 y 7,7 en una escala hedónica de 9 puntos, evidenciando una reacción hedónica positiva. Los nuevos híbridos presentaron puntajes de aceptabilidad simi-lares o superiores a los cultivares de referencia Tango, Gold Nugget, Murcott y Ortanique. A su vez, los resul-tados mostraron que el color naranja, la forma regular, la piel lisa, el dulzor, la jugosidad, el sabor intenso y el sabor típico a mandarina son características claves que determinan la aceptabilidad de las mandarinas, y que la apariencia externa no es un buen predictor del sabor. Los híbridos F3P8, F5P8 y F2P3 fueron descritos por los consumidores con términos sensoriales positivos, recibiendo valores de aceptabilidad similares o mayores que los cultivares de referencia como tango, reafirmando la importancia de la incorporación de estudios senso-riales en el contexto del mejoramiento genético. .-.-.-.-.-.-.-.-.-.-.-.-.-.-. RESUMO - As cultivares de tangerina apresentam grande diversidade na qualidade dos frutos e nas características senso-riais, juntamente com uma época de colheita prolongada. O melhoramento cítrico tem como foco a exploração de nichos sazonais por preços mais elevados, além de aspectos nutricionais e sensoriais, entre outros. Nesse contexto, a caracterização sensorial de diferentes híbridos e a identificação das características-chave do gosto do consumidor fornecem informações valiosas para os esforços de melhoramento e estratégias de marketing. Trabalhos anteriores mostraram que as características sensoriais e principalmente o sabor desempenham um papel fundamental na aceitação das tangerinas pelo consumidor. Dois estudos foram realizados durante a safra de tangerina (meio e final) aplicando perguntas do tipo cheque todos que se aplicam (CATA) com pelo menos 100 consumidores. As pontuações de aceitação geral dos híbridos e cultivares mais apreciados variaram entre 6,5 a 7,7 na escala hedônica de 9 pontos, evidenciando uma reação hedônica positiva. Os híbridos locais apresentaram valores semelhantes ou superiores aos cultivares de referência Tango, Gold Nugget, Murcott e Ortanique. Os resultados mostraram que a cor laranja, formato regular, maciez, doçura, sabor intenso y sabor tangerina e suculência típicos são os principais motivadores do gosto do consumidor por tangerinas e que a aparência externa não é um bom indicador da experiência de degustação da tangerina. Os híbridos F3P8, F5P8 e F2P3 foram descritos usando termos relacionados a características sensoriais positivas e receberam pontu-ações de gosto semelhantes ou superiores a cultivares de referência como o Tango, enfatizando a importância da ciência sensorial e do consumidor como parte integrante das estratégias de melhoramento.
650 $aCITRUS
650 $aFITOMEJORAMIENTO
650 $aSABOR
653 $aBreeding
653 $aCalidad de fruta
653 $aCitros
653 $aFlavor
653 $aFruit quality
653 $aMelhoramento
653 $aPLATAFORMA AGROALIMENTOS
653 $aQualidade do fruto
700 1 $aMOLTINI, A.
700 1 $aPINTOS, P.
700 1 $aLUQUE, E.
700 1 $aGONCALVEZ, L.
700 1 $aRIVAS, F.
700 1 $aALCAIRE, F.
700 1 $aARES, G.
773 $tAgrociencia Uruguay, 2021, vol. 25$gn.2, article E540. DOI: https://doi.org/10.31285/AGRO.25.540
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